Objective. The aim of the present study was to evaluate the effect of electromagnetic field (EMF) exposure during developmental period on parameters of oxidative stress and histopathology of testis and testosterone level in adult rat F 1 generation.Methods and study design. In treatment group pregnant rats were exposed to 3mT EMF, 50Hz for 21 days. The sham group contained pregnant rats under same condition, but out off the EM field. Pregnant rats in room were used as control group. After delivery, the blood samples of mothers for biochemical analyses of total antioxydant capacity (TAC) and malondialdehide (MDA) were provided. The male pups were kept until maturity, then their TAC, MDA and testosterone levels were analyzed, also their tests were removed for investigation of histopathology changes with light microscopy.Results. Biochemical analysis showed that TAC and MDA was significantly increased in pregnant rats in the treatment group when compared with the control group (p<0.05). In adult male of F 1 generation MDA was significantly increased in treatment group, but TAC and the testosterone level was significantly decreased in the treatment group as compared with the control group (p<0.05). Microscopic results revealed that in experimental group seminiferous epithelium contained many small irregular empty spaces as the sign of cellular sloughing, spermatogenic cells appeared to be disrupted. The nuclei of spermatogonia cells were heterochromatic, also dense of germinal epithelium and the number of spermatozoa was decreased.Conclusion. The results of this study suggest that pregnant rats exposure in EMF led to oxidative stress in adult male of F 1 generation and showed adverse effect on testosterone and spermatogenesis in adulthood which may produce subfertility.

The aim of the present study was to evaluate the effects of Electromagnetic Field (EMF) in the prenatal period on biomarkers of oxidative stress and fertility of F1 generation. Material and Methods: The pregnant rats divided to 3 group’s treatment (EMF exposure), Sham (off -EMF) and control. After delivery the blood sample of mothers provided and pups were kept until maturity age, and then their fertility was investigated. Results: CAT in treatment group was significantly decreased but no differences in GPX were observed. In treatment group decrease in multicellular embryos and abnormality in cleavage pattern was found. Conclusion: Exposure in EMF cause oxidative stress and reduce fertility.