Polymethyl methacrylate (PMMA) is a widely used material in prosthetics and is used to fabricate denture bases. The main disadvantage of this material is its polymerization shrinkage which causes clinical problems during use. The present study aimed to investigate and compare the microhardness, surface roughness, and water sorption of a commercial acrylic resin denture, which were processed by two different methods including conventional and pressure-packed injection molding techniques. A total of 60 polymethyl methacrylate samples were prepared in two groups: conventional acrylic resin (vertex) for the compression molding method and injection acrylic resin (vertex) for the injection molding method (10 samples of each material per test). The microhardness test was performed using a Vickers microhardness test device, the surface roughness test was performed by using a profilometer, and the water sorption test was performed using a digital scale. Data were analyzed using an independent sample t-test with Statistical Package for the Social Sciences (SPSS), version 17. The significant level was considered to be 0.05. According to the results, there was a significant difference between microhardness, surface roughness, and water sorption of the samples in the two groups. The results of the independent t-test showed that the microhardness of injection vertex acrylic resin samples was significantly higher than that of conventional pressure-packed vertex acrylic resin samples ( P value<0.05). Also, the surface roughness and water sorption of injection vertex acrylic resin samples were significantly lower than those of conventional pressure-packed vertex acrylic resin samples ( P value <0.05). According to the obtained results, denture fabrication by the injection molding method can improve the quality and durability of dentures due to the increased microhardness, the decreased surface roughness, and the decreased water absorption of the denture base compared with the conventional method.