Open-ended test is one of the diagnostic tests that can be used to measure misconceptions in students. The use of open-ended tests will give students the freedom to write their reasons for choosing answers to multiplechoice options. The purpose of this study is to identify misconceptions of 21st century biology learning material for prospective biology teacher students in Malang State University. The development model used is the ADDIE development model. The sample used was 26 students of prospective biology teacher at Universitas Negeri Malang in the 7th semester. The results of the open-ended test analysis for innovative biology learning materials, life-based learning, and 21st-century learning characteristics experienced misconceptions of 23%, 19%, and 18%. The misconceptions experienced by these students are still relatively low because the percentage of misconceptions is still in the range of 0-30%. Based on the results of the analysis it can be seen that there are still students who have a different understanding from the general concept of experts. Students are expected to be able to increase their understanding of concepts by creating learning tools that have been assigned in the form of concept maps/mind maps/resumes.
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