The aim of this work was to study the presence of mycobacteria in tissue samples from four cadavers fixed with formalin, and tissue samples from a recently deceased unpreserved individual, who had a history of human tuberculosis infection, undergoing a post mortem (cause of death not related to tuberculosis). All were examined for the presence of tuberculous lesions and the specific presence of Mycobacterium tuberculosis complex (MTC) and M. avium complex (MAC) members by microscopy, culture, and PCR analysis of four genomic elements (IS6110, mtp40, IS901, and IS1245). Microscopy examination after the Ziehl-Neelsen staining and culture examination for the presence of mycobacteria were negative in all 22 tissue samples from the four embalmed cadavers. PCR analysis of IS6110 and mtp40 was positive in tissue samples of tuberculous lesions from the lungs of two embalmed cadavers, and from intact kidney tissue of one of these cadavers. Microscopy and culture examinations of liver and spleen tissues from the unpreserved cadaver were positive for mycobacteria. PCR analysis, specific for M. avium subsp. avium, was positive in both tissue samples with, and without tuberculous lesions.

This work deals with the determination of undeclared or illicitly added sugar content in honey samples evaluated using the High-Performance Liquid Chromatography or HPLC with refractive index detection. Labelling of samples was also evaluated in accordance with current legislation. In a total of 21 samples of honey purchased in the fall of 2013, 13 samples were obtained from the regular shopping network, 2 samples were purchase in Health Food stores and 6 samples came directly from local beekeepers and were purchased at the Christmas Markets in Brno. We have determined the contents of fructose, glucose, sucrose, and oligosaccharides using the HPLC method. We have calculated the basic statistics such as the mean and standard deviation for each sample. Samples have been evaluated according to the Council Directive 2001/110/EC, which lays down limit values for the parameters of honey. Only four out of 21 honey samples complied with the requirements of Council Directive 2001/110/EC. These were three samples obtained from the regular shopping network and one obtained directly from the local beekeeper. Six samples did not meet the requirements for the sum of fructose and glucose, two samples could not be determined due to the failure to specify the honey type, and fourteen samples failed the requirement of sucrose content. We have further assessed whether honey samples comply with legislative requirements relating to this product or consumer misleading practices take place. Our analysed samples often lacked indication whether it is a floral honey or honeydew honey; this information was missing in eight out of 21 samples. Samples 5 and 9 did not mention the name of manufacturer. Sample 10 did not mention the country of origin.