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ine isolates of Verticillium dahliae, (VD 1 -VD 9 ) were isolated from roots and stems of wilted rose plants (1-2 years old) grown in Minia and Abo-Qurqas counties, Minia Governorate during summer, 2012. All isolates were able to induce wilt symptoms with different degrees of vascular discoloration on rose plants through 60 -90 days after planting. The maximum percentages (100%) of wilted plants were obtained due to using isolates VD 1 , VD 2 and VD 3 which were isolated from Minia county, followed by isolate VD 8 which was isolated from Abo Qurqas county (91.7%). All tested concentrations of Rizolex-T and Topsin-M decreased the linear growth of the four tested isolates. The fungitoxic effect was increased with increasing the fungicide concentration up to 400 ppm; that inhibited completely the growth of the tested fungi. Phos-root X and Formula X-1 stimulated the growth of the four tested isolates at the lowest rates, whereas the highest level, 3 ml/l, decreased the growth than control. Two bioagents, i.e., T. viride and B. subtilis were able to inhibit the mycelial growth of all tested pathogenic isolates. B. subtilis was effective in reducing the growth of the tested isolates where it gave 64.8 and 85.9% inhibition comparing to T. viride. The tested fungicides with different concentrations reduced significantly the percentage of infected plants comparing with control. Rizolex-T was more effective than Topsin-M in their efficiency. The maximum reduction of Verticillium wilt disease was recorded with using 3 ml/l of Phos-Root X and 2ml/l of Formula-X. Addition of the two tested bio agents one week before planting to soil significantly decreased the percentages of infected rose plants and disease severity under artificial infection with any of the tested isolates of Verticillium dahliae.

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eaf and pod spot disease of pea (Pisum sativum L.) caused by Septoria pisi Westend is considered as a common factor that reduces yield production. Three isolates of Septoria pisi were isolated from naturally infected leaves and pods of pea showing spots collected from Minia and Matai Provinces, Minia Governorate. Pathogenicity test indicated that these isolates were differed in their virulence; isolate S 1 was the most virulent one. Master B, Qena 1 and Qena 3 pea cultivars were susceptible to infection, whereas Jaguar and Palmoral cvs were more resistant. Pycnidia formation and sporulation of S. pisi, which are frequently used as propagules for infection and also are necessary to identify the species, can be scarce and are often reduced when the fungus is cultivated in vitro. A series of experiments were conducted to assess the effect of temperature, culture media, carbon and nitrogen sources on S. pisi growth and pycnidial production. Best results were obtained when fungal colonies were grown at 22±2 o C on oat meal, malt and pea dextrose of natural media and Czapek's medium from the synthetic media, in 12 hours-photoperiod at pH 6.5. The lowest growth and sporulation was recorded on basil and garlic extracts media. The best growth and spore formation were obtained when fructose, glucose, maltose and sucrose represented as the sole carbon sources, followed by raffinose, dextrin and starch. Pectin, sorbitol, manitol and sodium citrate proved to be poor sources of carbon for the growth of the tested fungus. Ammonium nitrate and ammonium sulphate mostly favored fungal growth. Minimum growth was observed when each of urea or asparagine was used as a sole nitrogen source. Peptone and the amino acid threonine were the best nitrogen sources for sporulation and forming pycnidia of S. pisi. Whereas, no spores or pycnidia were formed when each of sodium nitrite, cystine or cysteine was used as a sole nitrogen sources.