The ZnO NPS uses in many various aspects of life and has the ability to penetrate physiological barriers, and moving with blood circulation to different organs, which affects the health of the organism, so aimed the present studying to know the impact of ZnO-NPs of renal of male mice. Materials and Methods were used eighteen of Mus musculus male mice, the treated group injected (i.p) with ZnO NPS (300 mg/kg) at a period 30 day, while a group of control injected by 0.5 ml of 0.9 physiological solution. Urea, creatinine, and uric acid were measured as a biomarker indicates to renal function, Malondialdehyde was measured in the renal tissues to evaluated oxidative stress, apoptosis was also evaluated in renal tissues by measured the gene expression of caspase 8 and caspases 9. Results: ZNO NPS resulted in significant increase in renal function (Creatinine, Urea, and uric acid), a significant increase in Malondialdehyde, a significant decrease in caspase 9 in the renal while there's no significant difference in caspase 8. Conclusion: ZNO NPS was caused nephrotoxic, and It has an inhibiting effect of the intrinsic pathway of apoptosis of male mice.