Background: Decrease Anti-mullerian hormone and vitamin D was linked to increased risks of osteopenia and osteoporosis. Aim of the study: To find any association of Anti-Mullerian hormone and 25-hydroxyvitamin D serum levelswith bone minerals density test results in women. Material and Methods: The study was design as a cross-sectional; it includes one hundred forty five females, their age range from 23-68 years. These females attended Rheumatology department of Merjan Medical City in Babylon /Iraq through the period from September 2017 till March 2018.Hormonal profile (anti-mullarian hormone, and vitamin D) were assessed; and minerals density of the bone was measured by dualenergy x-ray absorptiometry for all participants. Results:The incidenceof osteoporosis and osteopenia in our studied population was(31, 72%, 23.45%) respectively and (44.83%) were with normal resultof bone mineral density study. Significant positivecorrelation (p<0.05) was found between Anti-Mullerian Hormone and Vitamin D serum concentrations with bone minerals densitystudy results. Conclusions:Vitamin D serum levels significantly correlates with Anti-mullerian serum levels in patients withabnormal bone mineral density.