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We present an overview of fission chamber’s functioning modes, theoretical aspects of the nonnegative matrix factorization methods, and the opportunities that offer neutron data processing in order to achieve neutron flux monitoring tasks. Indeed, it is a part of research project that aimed at applying Blind Source Separation methods for in-core and ex-core neutron flux monitoring while analyzing the outputs of fission chamber. The latter could be used as a key issue for control, fuel management, safety concerns, and material irradiation experiments. The Blind Source Separation methods had been used in many scientific fields such as biomedical engineering and telecommunications. Recently, they were used for gamma spectrometry data processing. The originality of this research work is to apply these powerful methods to process the fission chamber output signals. We illustrated the effectiveness of this tool using simulated fission chamber signals.