The novel mode of action and newer pesticides product molecules is a promising component for agriculture management programs. For this argument some acaricide toxicity evaluations were completed on the predatory mite Phytoseiulus persimilis Athias-Henriot (Acari: Phytoseiidae) in laboratory rearing for two generation under acaricide sublethal effect. Toxicity results showed that chlorfenapyr was the most toxic acaricide for Tetranychus urtica Koch and etoxazole was the lesast and for P. persimilis, etoxazole was the most toxic and fenproxymat was the least. Relative toxicity recorded was higher in etoxazole and fenproxymat (7.916 and 5.058 times) respectively. Selectivity data approved that abamectin was the most selective acaricide and etoxazole was the least (8.24 and 0.268) respectively. Effect on first generation of P. persimilis growth fertility and productivity declined, consequently, R0 and rm values where in LC25 attained values ranged from 0.59 to 0.66 for cyflumetofene and hexythiazox, ri data ranged from 0.29 to 0.36 for fenproxymate and cyflumetofene and R0 ranged from 0.53 to 6.0 for cyflumetofene and hexythiazox respectively. But in the second generation similar results attained because declined toxicity where rm in LC25 was from 0.93 and 1.13 for fenproxymat and etoxazole and in LC50 was 0.90 and 1.18 for cyflumetofene and hexythiazox respectively. A high effect on No of eggs /female/day produced, shortened in total time of the generation at LC50 level of treatment more than LC25, and sex ratio resulted more male number emerged than female. The high acaricide concentrations used can kill more T.urtica than P.persimilis.

The Pink Bollworm Pectinophora gossypiella (Saunders) is a devastating pest in Egyptian cotton field. Insecticide control failure may be happen due to its biological features of hide inside bolls and rarely exposed to insecticide application. Thus, a bulk of bolls infested with larvae collected from fields of Sharkia and Benysuef governorate. Moreover, exposed to py, Op sub lethal concentrations in laboratory bioassay. Toxicity features as LC50, LC90 and the slope, and RR was completed. Whereas the common target site of OP and pyrethroid is the acetylcholinesterase enzyme. Thus, the gene Ace1 encoding AChE relative expression was investigated using molecular marker of real-time PCR procedures via the steps of generating data was RNA isolation and characterization, cDNA synthesis, then generating normalization factors, and Ct data attained and analyzed using 2-ΔΔCT method to quantify the gene expression quantitatively of insecticide treated larvae tissue extracted. Data showed the most expressed ratio of samples was cypermethrin followed by deltamethrin compared with the control and the least was malathion and chlorpyrifos, thus, the most effective insecticide was malathion and the least were cypermethrin according to ct values. Hierarchical clustering combined with Heat map and principle component analysis based on various clustering systems and algorithm as distance measures were completed. Validation of reference gene in addition to gene expression stability analyzed by the three optimal gene finders and showed that deltamethrin and cypermethrin treated genes having lower mean weights and considered transcriptionally stable and ideal reference genes.