Though the biological effects of human placental extract have been widely studied, it has limited availability and its use poses ethical problems. Thus, domestic animal placental extracts are suggested as alternatives. In this study, the protective effect of sheep placental extract (SPE) on concanavalin A (Con A)-induced liver injury was investigated. BALB/c mice were randomly divided into six groups, including one normal group and five experimental groups, which received different oral doses of SPE (0, 5, 10 and 50 mg/kg) or a mixture of amino acids for 3 days before Con A injection. Compared with Con A-induced model group, the SPE administration significantly decreased serum aminotransaminase activity, alleviated pathological changes, recovered liver antioxidant capacity and prevented the increase of nitric oxide. Secretion of pro-inflammatory cytokines in serum decreased and mRNA expression of hepatic intercellular adhesion molecule-1, interferon-inducible chemokine 10 and inducible nitric oxide synthase were downregulated, while B-cell lymphoma-2 expression increased. The administration of amino acids mixture had no significant effect in most measurements compared with the model group, which indicated proteins and peptides, rather than individual amino acid, were largely responsible for the bioactivity of SPE. The results indicate SPE has potential therapeutic effects against immune-mediated hepatitis.

No abstract

The glucose analog, 2-deoxyglucose (2-DG), specifically inhibits glycolysis of cancer cells and interferes with the growth of cancer cells. However, the excellent water solubility of 2-DG makes it difficult to be concentrated in tumor cells. In this study, a targeted nano-pharmacosome was developed with folic acid-modified 2-DG (FA-2-DG) by using amino ethanol as a cleavable linker. FA-2-DG was able to self-assemble, forming nano-particles with diameters of 10–30 nm. The biological effects were evaluated with cell viability assays and flow cytometry analysis. Compared with a physical mixture of folic acid and 2-DG, FA-2-DG clearly reduced cell viability and resulted in cell cycle arrest. A computational study involving docking simulation suggested that FA-2-DG can dock into the same receptor as folic acid, thus confirming that the structural modification did not affect the targeting performance. The results indicated that the nano-pharmacosome consisting of FA-2-DG can be used for targeting in a nano-drug delivery system.

An electrochemical sensor using silver nanowires (AgNWs)-doped with a zeolite-like metal–organic framework (ZIF-67) was developed for highly sensitive and stable determination of folic acid (FA). The ZIF-67/AgNWs nanocomposite was prepared by a one-step reaction via a template method and drop-coated onto the surface of a screen-printed carbon electrode (SPCE) to form a ZIF-67/AgNWs@SPCE electrochemical sensing platform. The electrochemical square wave voltammetry (SWV) curve for this sensing platform was measured in an electrolyte solution containing FA under the optimum experimental conditions. The redox peak current of FA (IFA) increased with increases in the FA concentration (CFA). There was a linear relationship between IFA and CFA in the range of 0.1 μM to 10 μM, and the determination limit was 30 nM. The ZIF-67/AgNWs@SPCE was used as an electrochemical sensor for FA which maintained a good stability over 7 days and showed good determination performance in real samples with a high recovery rate (100.9–102.1%, n = 6).