A UV hyperspectral instrument was designed with a polarization measurement channel for real-time in-orbit polarization correction to reduce the influence of polarization on the detection accuracy of atmospheric radiation. One of the prerequisites for in-orbit polarization calibration is accurately calibrating the instrument’s polarization properties in the laboratory. This study first introduces the calibration method and measuring device of the polarization characteristics of the ultraviolet (UV) hyperspectral detector and conducts a polarization calibration test of the instrument. The two main error sources introduced by the calibration device were emphatically analyzed, and the correction method of the error sources was deduced theoretically. Finally, the polarization calibration results of the UV hyperspectral detector were corrected, and the uncertainty analysis of the corrected calibration results was about 1.4%, which provides effective ground polarization calibration data for the on-orbit polarization correction of the instrument.