Catharanthus rosea is an important ethno medicinal plant rich in bioactive compounds mainly alkaloids used widely in treatment of cancer disease. The aim of present study was to determine the total phenol, flavonoid, tannin and alkaloid content in leaf stem and root parts of Catharanthus rosea in benzene, methanol, aqueous, chloroform and petroleum ether extracts by spectrophotometric method. Phytochemical analysis showed presence of saponin, tannin, terpenoid, flavonoid, cardiac glycoside, alkaloid, steroid, quinones and phenolic compounds whereas protein was found absent. The total phenol, flavonoid, tannin, and alkaloid content of plant in different extracts were determined in comparison to standard gallic acid, rutin, tannic acid and quercetin respectively. The higher concentration for phenol was found in benzene leaf extract (0.65±0.02) µg/ml, flavonoid in chloroform leaf extract (0.62±0.02) µg/ml, tannin in methanol root extract (21±0.01) µg/ml and alkaloid in benzene leaf extract (0.61±0.03) µg/ml. The presence of such metabolites indicates therapeutic importance of plant.

Senna alata is an ornamental flowering plant also known as ringworm bush and candle bush belongs to Leguminaceae family and used mainly as antifungal agent .The plant is pharmacological significant and used as antibacterial, antioxidant, anti-cancerous, anti-mutagenic, antifungal, anti-diabetic and antiviral activities. Leaves of the plant have anti-parasitic activity used in treatment of eczema, ringworm, asthma, bronchitis and in poisonous insect bites, bark of the plant is used in treatment of skin diseases. In the present study chloroform, methanol, petroleum ether and aqueous extracts were used for phytochemical analysis, TLC profiling and antioxidant activity of Senna alata. The phytochemical analysis of Senna alata indicated presence of tannins, saponins, flavonoids, alkaloids, terpenoids, steroids, cardiac glycosides and quinones in Senna alata. In TLC analysis maximum 6 bands were found in leaf extract, 5 bands in stem extract and 4 bands were found in root methanol extract. The plant showed good antioxidant activity in methanol and aqueous based leaf, stem and root extract which may be due to the presence of flavonoids and phenolic compounds. The plant has potent antioxidant and antimicrobial properties so characterization of bioactive molecules of the plant should be beneficial for formulation of drugs.

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