A new specific, precise, accurate and robust RP-HPLC method has been developed for the simultaneous determination of Hydroquinone and Monobenzone in a pharmaceutical cream formulation. The chromatographic separation was carried out at Analytical technology HPLC instrument (Software: HPLC Work Station) equipped with Deuterium lamp as detector, HPLC pump and manual injecting facility programmed at 20 μL capacity per injection was used. The stationary phase was Zodiac Column C 18 (250 mm x 4.6 mm, 5μm) at ambient temperature. The mobile phase was Acetonitrile: Triethylamaine Buffer (60:40) adjust pH 3.5 by 10% orthophosphoric acid. Detection was carried out at 230nm using UV Detector. The flow rate was 1.0mL/min and retention time was about 3.3mins and 5.1mins for Hydroquinone and Monobenzone. The linearity was obtained in the concentration range of 24-64µg/mL and 30-70µg/mL for Hydroquinone and Monobenzone respectively. Mean percentage recoveries were 99.89% for Hydroquinone and 99.57% for Monobenzone. The LOD of Hydroquinone and Monobenzone was found to be 1 μg/mL and 2.0μg/mL whereas the LOQ was 5μg/mL and 10 μg/mL respectively. The assay values of both the analytes was found to be well within the limits that is 100.05% and 99.55%for Hydroquinone and Monobenzone respectively. Percentage relative standard deviation of percent assay values for replicate sample preparation was 1.09% for Hydroquinone and 0.96% for Monobenzone. The method was robust with respect to change in flow rate, and composition of mobile phase.

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