Nowadays, the possibility of substituting gasoline fuel with biofuels is examined by numerous researchers. This paper discusses the numerical study of the biofuel model (methyl decanoate). The Fluent code was used to validate the comparison between the non-premixed gasoline (n-decane) and biofuel model (methyl decanoate) combustion in the same conditions. The turbulence model used was the realizable k- model. The aerothermochemistry equations and the transport model of chemical species (Eddy-dissipation) were implemented in the combustion reaction to develop the velocity, pressure, temperature, energy, enthalpy, the turbulence dissipation rate, the kinetic energy of the turbulence, and the mass fraction of the species. The results showed that the CO 2 and NO X contents of methyl decanoate are 5.7% and 11.03% respectively higher than those of decane.