Abstract. Deep observation (∼113 hrs) of the Cygnus region at TeV energies using the HEGRA stereoscopic system of aiř Cerenkov telescopes has serendipitously revealed a signal positionally inside the core of the OB association Cygnus OB2, at the edge of the 95% error circle of the EGRET source 3EG J2033+4118, and ∼0.5• north of Cyg X-3. The source centre of gravity is RA α J2000 : 20 hr 32 m 07 s ± 9.2 s stat ± 2.2 s sys , Dec δ J2000 : +41• 30 30 ± 2.0 stat ± 0.4 sys . The source is steady, has a post-trial significance of +4.6σ, indication for extension with radius 5.6 at the ∼3σ level, and has a differential power-law flux with hard photon index of −1.9 ± 0.3 stat ± 0.3 sys . The integral flux above 1 TeV amounts ∼3% that of the Crab. No counterpart for the TeV source at other wavelengths is presently identified, and its extension would disfavour an exclusive pulsar or AGN origin. If associated with Cygnus OB2, this dense concentration of young, massive stars provides an environment conducive to multi-TeV particle acceleration and likely subsequent interaction with a nearby gas cloud. Alternatively, one could envisage γ-ray production via a jet-driven termination shock.

No abstract

Terrestrial tardigrades are often found in the lichens and mosses growing on trees and rocks. The assertion that tardigrades in these habitats are very patchy in their distribution has rarely been backed by quantitative sampling. This study assesses spatial variability in tardigrade populations inhabiting small patches (0.1 cm 2 to over 5 cm 2 ) of moss and lichen on trees and rocks at three sites in the United States of America. Tardigrades were collected from four replicate rocks in the Ouachita Mountains of Arkansas, with 30 lichen patches collected on two adjacent boulders and 20 moss patches on a second pair of boulders. In Fort Myers and in Citrus Springs, Florida, 30 lichen patches per tree were collected from two pairs of trees. The tardigrades in each sample were extracted, mounted, identified, and counted. The variation in tardigrade abundance among lichen or moss patches within rocks or trees was very high; the only consistent pattern was that very small patches usually lacked tardigrades. Tardigrade diversity and abundance also varied greatly within sites when lichens and mosses of the same species from different rocks and trees were compared (in the most extreme case one tree had numerous individuals of two tardigrade species present while the other had almost no tardigrades). The results of this quantitative sampling support the assertion that tardigrades are very patchy in distribution. Given the considerable time investment required for the quantitative processing of tardigrade samples, this high spatial variability in tardigrade diversity and abundance requires that researches testing ecological hypotheses about tardigrade abundance check variability before deciding how many samples to take.

This paper provides a comprehensive list of the freshwater and terrestrial tardigrade fauna reported from the Americas (North America, South America, Central America and the West Indies), their distribution in the Americas, and the substrates from which they have been reported. Data were obtained from 316 published references. Authors' identifications were accepted at face value unless subsequently amended. Taxa were assigned to sub-national units (states, provinces, etc.). Many areas, in particular large portions of Central America and the West Indies, have no reported tardigrade fauna.        The presence of 54 genera and 380 species has been reported for the Americas; 245 species have been collected in the Nearctic ecozone and 251 in the Neotropical ecozone. Among the tardigrade species found in the Americas, 52 are currently considered cosmopolitan, while 153 species have known distributions restricted to the Americas. Based on recent taxonomic revision of the genus Milnesium, the vast majority of records of M. tardigradum in the Americas should now be reassigned to Milnesium tardigradum sensu lato, either because the provided description differs from M. tardigradum sensu stricto or because insufficient description is provided to make a determination; the remainder should be considered Milnesium cf. tardigradum.        Most terrestrial tardigrade sampling in the Americas has focused on cryptogams (mosses, lichens and liverworts); 90% of the species have been collected in such substrates. The proportion of species collected in other habitats is lower: 14% in leaf litter, 20% in soil, and 24% in aquatic samples (in other terrestrial substrates the proportion never exceeds 5%). Most freshwater tardigrades have been collected from aquatic vegetation and sediment. For nine species in the Americas no substrates have been reported.

Mainly because of the problems with species delineation, the biogeography of microscopic organisms is notoriously difficult to elucidate. In this contribution, variable nuclear and mitochondrial DNA markers were sequenced from individual specimens representing the Echiniscus virginicus complex that are morphologically indistinguishable under light microscopy (five populations from the temperate Eastern Nearctic and 13 populations from the subtropical and tropical zone). A range of methods was used to dissect components of variability within the complex (Bayesian inference, haplotype networks, Poisson tree processes, automatic barcode gap discovery delineations, principal components analysis and ANOVA). We found deep divergence between the temperate Eastern Nearctic E. virginicus and pantropical Echiniscus lineatus in all three genetic markers. In contrast, intraspecific genetic variation was very low, regardless of the geographical distance between the populations. Moreover, for the first time, statistical predictions of tardigrade geographical distributions were modelled. The factor determining the allopatric geographical ranges of deceptively similar species analysed in this study is most likely to be the type of climate. Our study shows that widespread tardigrade species exist, and both geographical distribution modelling and the genetic structure of populations of the pantropical E. lineatus suggest wind-mediated (aeolian) passive long-distance dispersal.