A review of using Griess Reaction method to determine nitric oxide production in biological systems.

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Calibration of nitric oxide (NO) sensor is a key step to measurement of NO. Currently there are three methods to calibrate electrochemical NO sensors. However, there are many problems associated with these techniques, especially for calibration of microsensors. This article describes a novel method for calibration of NO microsensors, which has the advantage of being simple, reproducible, wide range and suitable for all kind of NO electrochemical sensors, especially for the commercial available integrated NO microsensors such as ISO-NOP30. This method is based on quantitative release of NO from decomposition of SNAP trigged by Cu . Using of monovalant copper, as described in the present study, represents a major methodological advancement in the quantitative release of NO from SNAP. The kinetic decomposition of SNAP, effect of Cu concentration, pH of the solution, temperature and oxygen concentration in the solution on the release of NO are discussed. Compared with other calibration methods, this method is simple, convenient, reliable, accurate.