Calcium (Ca2+) signaling is pivotal in transmission of information in the cell. Various Ca2+ sensing molecules work to sense and relay the encrypted messages to the intended targets in the cell to maintain this signal transduction. CBL-interacting protein kinases (CIPKs) are crucial components of Ca2+ signal transduction during various abiotic stresses. Although there are intron rich CIPKs in the plant genome but very little has been reported about their alternative splicing. Moreover the physiological significance of this event in the Ca2+ signaling is still elusive. Therefore in this study, we have selected CIPK3, which has highest number of splice variants amongst Arabidopsis CIPKs. Expression profiling of five splice variants of CIPK3 by qRT-PCR in four Arabidopsis thaliana ecotypes revealed preferential transcript accumulation but similar subcellular localization of the variants and interaction with similar CBLs. ABA and drought treatment resulted in the higher accumulation of the alternately spliced transcripts of CIPK3 in Arabidopsis ecotype Wassilewkija. The transcripts of CIPK3.1 and CIPK3.4 are relatively more induced compared to other alternative splice variants. Out of four splice variants studied, we found CIPK3.1 and CIPK3.2 showing preference for ABR1, a previously reported interactor of CIPK3. We conclude that the differential expression and choice of downstream partner by CIPK3-splice variants might be one of the mechanisms of Ca2+ mediated preferential regulation of ABA and other stress signals.

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Voltage-dependent anion channels (VDACs) are conserved proteins of the mitochondria. We have functionally compared Arabidopsis VDACs using Saccharomyces cerevisiae Dpor1 and M3 yeast system. VDAC (1, 2, and 4) were able to restore Dpor1 growth in elevated temperature, in oxidative and salt stresses, whereas VDAC3 only partially rescued Dpor1 in these conditions. The ectopic expression of VDAC (1, 2, 3, and 4) in mutant yeast recapitulated the mitochondrial membrane potential thus, enabled it to maintain reactive oxygen species homeostasis. Overexpression of these VDACs (AtVDACs) in M3 strain did not display any synergistic or antagonistic activity with the native yeast VDAC1 (ScVDAC1). Collectively, our data suggest that Arabidopsis VDACs are involved in regulating respiration, reactive oxygen species homeostasis, and stress tolerance in yeast.

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STI/HOP functions as a co-chaperone of HSP90 and HSP70 whose molecular function has largely been being restricted as an adaptor protein. However, its role in thermotolerance is not well explored. In this article, we have identified six members of the TaSTI family, which were named according to their distribution on group 2 and group 6 chromosomes. Interestingly, TaSTI-2 members were found to express higher as compared to TaSTI-6 members under heat stress conditions, with TaSTI-2A being one of the most heat-responsive member. Consistent with this, the heterologous expression of TaSTI-2A in Arabidopsis resulted in enhanced basal as well as acquired thermotolerance as revealed by the higher yield of the plants under stress conditions. Similarly in the case of rice, TaSTI-2A transgenics exhibited enhanced thermal tolerance. Moreover, we demonstrate that TaSTI-2A interacts with TaHSP90 not only in the nucleus but also in the ER and Golgi bodies, which has not been shown till now. Additionally, TaHSP70 was also found to interact with TaSTI-6D specifically in the cytosol. Thus, these data together suggested that the TaSTI family members might play different roles under heat stress conditions in order to fine-tune the heat stress response in plants.