Nurse is someone who has a task as a medical asisstant in the hospital. They work for eight hours per day in serving patients and helping out the doctors such as, wound cares, infusions, bathing patients, injecting medical substances, giving medications, moving patients, changing patient clothes, etc, creating a lot of work for them. According to survey of the Indonesian National Nurses Association there are 50.9% of nurses experiencing work stress, dizziness, and fatigue because of high workloads. Therefore, it is necessary to be improved. Objective of this study is to analyse the mental workload of nurses and develop few of recommendations to reduce the mental workload. National Aeronautical and Space Administration Task Load Index (NASA-TLX) is used to evaluate the mental workload based on six variables: mental demand, physical demand, temporal demand, own performance, effort, and frustation level. While the developed improvement uses the participatory ergonomic method which involve nurse, doctor, ergonomist, and designer as stakeholder by implementing a group discussion to determine criteria of enhancement. Statistical analysis is applied to verify the hypothesis. Result of this study shows that the score of NASA-TLX is 62.46, which means the task is overload. As for the result, participatory ergonomic is verified at 5% of significant levels that requires some improvements, including but not limited to administrative activities in the form of making action invoices, physical activity that causes spinal cord injuries was lifting the patients and bending while injecting the patient, and work shift system where morning and afternoon shift longer than night shift.