Okilactomycin, a novel antibiotic, was isolated from the culture filtrate of a strain of actinomycetes. The producing organism, strain YP-02908L, was identified as Streptomyces griseoflavus subsp. zamamiensis subsp. nov. The antibiotic was extracted with ethyl acetate and purified by silica gel column chromatography. It was obtained as colorless prisms from a dichloromethane solution. It exhibited weak antimicrobial activity against Grampositive organisms in vitro. It also exhibited antitumor activity against Ehrlich ascites carcinoma in vivo. The apparent molecular formula of okilactomycin was determined as C24H32O6.It is a new memberof the lactone group antibiotics. 1475 In the course of our screening for new antibiotics, a novel antibiotic, okilactomycin, was found. The antibiotic was produced by Streptomyces griseoflavus subsp. zamamiensis subsp. nov. It exhibited weak antimicrobial activity against Gram-positive organisms and antitumor activity agaist Ehrlich ascites carcinoma. In this paper, we describe the taxonomy of the producing organism, the fermentation, isolation and characterization of the antibiotic. Materials and Methods Chemicals Chemicals employed were as follows: Kieselgel 60 and TLC plates Silica gel 60 F254 (0.25 mm thickness) from E. Merck, Darmstadt, FRG; Sephadex LH-20 from Pharmacia Fine Chemicals, Sweden; mitomycin C from KyowaHakko Kogyo Co., Ltd., Japan. All other chemicals were of analytical grade. Components for Media Components for media employed were as follows :

Hatomamicin,a newalkaloid antibiotic, wasisolated from the culture filtrate of a strain of Saccharopolyspora. The antibiotic was extracted with EtOAcand purified by silica gel column chromatography. The free alkaloid was obtained as pale yellowish prisms from CH3CN solution. It exhibits antimicrobial activity against Gram-positive organisms. The apparent molecular formula of hatomamicinwas determined to be C22H31NO5. The structure has been established by a combinationof spectroscopic and X-ray crystallographic studies.In the course of our screening for new antibiotics, a new alkaloid antibiotic hatomamicin was found. The antibiotic was produced by a strain of Saccharopolyspora. The strain YL-O358M was isolated from a soil sample collected at Hatoma Island, Okinawa Prefecture, Japan. The type strain has been deposited at the Fermentation Research Institute, Agency of Industrial Science and Technology, Japan, and assigned as Saccharopolyspora sp. YL-O358Mwith the accession No. FERMP-8862.Taxonomic studies of the strain are now in progress.The fermentation, isolation, physico-chemical properties, structure determination and biological properties of hatomamicin are described in this paper. Fermentation A loopful of well-grown agar slant culture of strain YL-O358M was inoculated into a seed medium (60 ml) containing dextrin 2.0 %, glucose 0.5 %, Polypepton 0.5 %, yeast extract 0.5 %, corn steep liquor 0.5%, brain heart infusion 0.52%, and CaCO3 0.2% (pH 8.0) in a 500-ml Erlenmeyer flask, and cultured at 28°C on a rotary shaker with 7.6cm throw at 200rpm for 48 hours. The seed culture was then transferred at the rate of 3.0% to 80 liters of the production medium in a 100-liter jar fermenter and cultiva- å¡ pH, à" hatomamicin.

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