There are several researches that have been done to improve the life among tetraplegia. It has become an attractive research field in the rehabilitation engineering because the eye movement have abilities as a communication tool for disabled people. However, the previous research has not much provided an appropriate design for the user among tetraplegia. Motivated from that, a new design of eye movement data acquisition kit has been developed. This paper aims to describe the design of eye movement data acquisition kit for the user among tetraplegia based on the proper electrode positions, the prototype as well as the signal conditioning circuits. Then, this EOG kit was used to acquire the eye signals for eye movement in the left and right direction. The eye movement data was obtained from the kit, which can be used as a significant communication tool among tetraplegia. The results show that the kit equipped with a proper signal conditioning is able to acquire the eye movement signal.