Purpose Having an infant in the neonatal intensive care unit(NICU) is highly stressful and it implicated in mother infant bonding. This study investigated the relationship between mothers’ dispositional mindfulness and level of stress and quality of bonding.Methods Self-report measures of Dispositional Mindfulness, Parental Stressor Scale, and the Postpartum Bonding Questionnaire were administered to 120 mothers with newborns admitted in the NICUs in three hospitals in Qom city in Iran. The data analysis was carried out by using a hierarchical multiple regression.Results Mean score of stress, bonding and mindfulness were 114.29±27.33, 67.77±5.38, and 126.27±22.39 respectively. There was a significant inverse relationship between total mindfulness score and mother’s parental stress. Also, high scores of stress were associated with greater impairment in bonding. Observing and acting with awareness led to better mother to infant bonding.Conclusions Improvements in mindfulness could help mothers to reduce stress and better bonding whit their infants.