Border disease is one of the serious viral disease responsible for worldwide significant economic losses in sheep and goats. The objectives of the current study were to verify the seroprevalence of border disease virus (BDV) using indirect ELISA-kit, to investigate the persistent infection (PI) using the antigen ELISA (Ag-ELISA) kit for local breeds of sheep and goats in Mosul city. A total of 364 blood samples (264 sheep and 100 goats) of 20 local Awassi sheep and Shami goat herds with ≥1.5 years old age were collected from different regions of Mosul city. The sampling areas have no vaccination history against BDV, The seroprevalence of BDV was significantly higher in sheep 46.9% (124/264) than in goats 16% (16/100) (P<0.05). The prevalence of PI of border disease in sheep was 1.4% (2/140), but not detected in goats. This is a preliminary study of BDV in Mosul city, indicating that BDV is highly prevalent in sheep and goats of this region.