Twenty Egyptian buffaloes were housed in shaded open yards and maintained under the same managerial and environmental conditions. Buffaloes were divided into two groups at the end of the first lactation season according to their lactation period (LP). The first group (G1) was lactated more than seven months (225 days on average) and the second group (G2) was lactated less than seven months (175 days on average).Milk samples were collected once weekly for each lactating buffalo at 6:00 AM and 6:00 PM and then mixed. Prolactin (PRL) and Insulin-like growth factor-1 (IGF-1) were determined during lactation period. There was a significant difference between G1 and G2 but there was non-significant difference between the stage average and the same trend was recorded in milk fat (F%). On the other hand there was a significant different in sold not fat (SNF%) in stage average.There was a high positive and significant correlation among milk yield and total solids (T.S %), F % and water (%). There was positive and significant correlation among milk yield, S.N.F (%) and Lactose (%).in the two lactating groups (G1 and G2). It was noticed that the concentration of IGF-1 was higher in G1 than that in G2 during the three stages of lactation by 40.21, 22.35 and 12.86 (%), respectively. PRL concentration in G1 was slightly higher in early and mid-lactation than that in the late lactation. There was a positive and significant correlation among PRL and T.S, Fat and water. There was high positive significant correlation among IGF-1, T.S, SNF, Ash, lactose and water.

Twenty Egyptian buffaloes were divided into two groups at the end of the first lactation season according to their lactation period (LP). The first group (G1) was lactated more than seven months (225 days on average) and the second group (G2) was lactated less than seven months (175 days on average). Body measurements were measured three times, at the beginning of the lactation period, after three months of onset lactation and at the beginning of the dry period for each buffalo cow. The body condition score (BCS) was measured during lactating season. BCS system was developed using ultrasonographic measurements of body fat reserves. The average of total milk yield (TMY) was significantly higher in G1 than that in G2 by 36.22%. The higher shoulder height was recorded in mid lactation period in G1. There were significant differences between groups in all skeletal check points. There was a negative correlation between milk yield and chest circle but there was a positive correlation between milk yield and ultra-sonographic fat thickness in lumbar vertebrae and chest.