Anthocyanins are components of the flavonoid group with different properties, such as antidiabetic properties. This study aimed to isolate anthocyanin from Berberis integerrima Bunge fruits and evaluate α-amylase and α-glucosidase inhibition by this mentioned anthocyanin. The anthocyanin of Berberis integerrima fruit was isolated using column chromatography, and the antidiabetic properties of the anthocyanin were determined by the levels of α-amylase and α-glucosidase inhibition. Km and Vmax were also evaluated using the GraphPad Prism 7. The results of this study showed that the anthocyanin content of the fruit extract was 14.36 ± 0.33 mg/g, and following purification, this amount increased to 34.51 ± 0.42 mg/g. The highest of α-glucosidase inhibition was observed in the purified anthocyanin with IC50 = 0.71 ± 0.085 mg/ml, compared to acarbose as the baseline with IC50 = 8.8 ± 0.14 mg/ml, p < 0.0001 . Purified anthocyanin of the mentioned fruit with IC50 = 1.14 ± 0.003 mg/ml had the greatest α-amylase inhibition, which was similar to acarbose as the standard with IC50 = 1 ± 0.085 mg/ml, p < 0.05 . The inhibition of α-glucosidase and α-amylase by purified anthocyanin showed uncompetitive inhibition, and the enzyme inhibition by unpurified anthocyanin showed mixed inhibition. The obtained findings showed that Berberis integerrima fruit can be mentioned as a source of anthocyanin with antidiabetic properties.

Background Diabetes mellitus is a type of metabolic disease characterized by elevated blood sugar. The main strategy for its treatment is to inhibit carbohydrate-hydrolyzing enzymes, including α-amylase and α-glucosidase. The aim of the present study was to evaluate the efficacy of Salvia extracts in inhibiting diabetes marker enzymes. Materials and methods This experimental study was performed in vitro. The studied plants included Salvia mirzayanii, Salvia macilenta, Salvia officinalis and Salvia santolinifola and inhibitory effects of their methanolic and dichloromethane extracts were investigated. After calculating the percentage of α-amylase inhibition and IC50 of the extracts, Km and Vmax were also determined using prism7.Statistical analysis was performed employing with Graph Pad instat3 software. Results The results here in revealed that methanol extracts of Salvia santolinifola (with IC50 = 54.72 ± 9.6 μg / ml) and Salvia officinalis with (IC50 = 54.87 ± 5.7 μg / ml) and dichloromethane extract of Salvia officinalis with (IC50 = 71.20 ± 14.3 μg / ml) had the greatest inhibitory effect on α-amylase comparing to acarbose with (IC50 = 42.94 ± 3.8 μg / ml) as a standard. Tukey test results showed that there is a significant difference between IC50 of acarbose comparing to methanol extract of Salvia mirzayanii and dichloromethane extracts of Salvia mirzayanii and Salvia santolinifola with P value ˂0.001 in α-amylase inhibition. Conclusion The extracts had significant inhibitory effects on α-amylase inhibition. Among the extracts of the studied species, methanol extract of Salvia santolinifola demonstrated the greatest inhibitory effect on α-amylase.

Background: Diabetes mellitus is believed to be the most serious metabolic disease. One of the treatments for diabetes is to delay glucose uptake by inhibiting carbohydrate-hydrolyzing enzymes. Alpha-glucosidase inhibitors delay glucose uptake. Objectives: The present study was conducted aiming to evaluate the efficacy of Salvia extracts in inhibiting diabetes marker enzymes and their effects on the treatment of diabetes. Methods: This experimental study was performed in vitro. The studied plants included Salvia macilenta and Salvia officinalis. The inhibitory effects of their dichloromethane and methanol extracts were also investigated. After calculating the percentage of inhibition and IC50, Km and Vmax using GraphPad Prism 7 were also calculated. The statistical analysis was performed employing GraphPad Instat 3 software. Results: The results herein showed that the greatest inhibitory effect on alpha-glucosidase belonged to the methanol extract of S. macilenta with IC50 = 8.73 ± 0.26 mg/mL compared to that of acarbose with IC50 = 8.82 ± 0.14 mg/mL as a standard. The IC50 of dichloromethane extract of S. officinalis was 8.95 ± 0.23 mg/mL. Conclusions: The extracts had significant inhibitory effects on alpha-glucosidase. However, methanol extract of S. macilenta and dichloromethane extract of S. officinalis demonstrated the greatest inhibitory effects on alpha-glucosidase compared to acarbose as a standard.