Fatty acids, sterols and tocopherols profile of Pulicaria incise, Diplotaxis harra and Avicennia marina grown wild in Egypt were investigated. Linoleic acid followed by palmitic and oleic acids were the major fatty acids in P. incise. Oleic followed by stearic were the main acids in D. harra, while palmitic followed by oleic were the major fatty acids found in A. marina. Sterol markers of P. incise were campesterol followed by stigmasterol and b-sitosterol. In D. harra b-sitosterol followed by stigmasterol and D5-avensterol were the major sterols, while campesterol followed by b-sitosterol and D7-avensterol were main sterols of A. marina. Tocopherols were also estimated in high levels, wherein d-tocopherol was the main tocopherol in all plants. Antioxidant properties of methanolic extracts of the plants were compared with methanolic solution of butylated hydroxyanisole (BHA), butylated hydroxytoluene (BHT) and ascorbic acid during thiocyanate assay. Moreover, methanolic extracts of the whole plants exhibited strong antiradical potential against DPPH radicals. Information provided by the present study is of importance for further investigations of P. incise, D. harra and A. marina and utilization of these plants as a raw material of bioactive compounds.