A different squid species was caught with a trawling net at the coordinates of 36o 39'.431'' N; 31o 9.841''E and a depth of approximately 600-650 m in the Gulf of Antalya. In the identification of species, this squid species was determined to be Histioteuthis reversa (Verrill, 1880). This is the second record for the squid species in the Mediterranean Coast of Turkey and the first record in the Gulf of Antalya.Keywords: Gulf of Antalya; Histioteuthis reversa; Mediterranean; Record
A detailed study of the Turkish endemic Gypsophila pilulifera were presented by both morphological and molecular investigations of several sites (each one divided in four biotopes). The characteristics were investigated using statistic analyses, i.e. t-Test and One-way ANOVA tests, as well as Discriminant analysis (á=0.05). Some differences were detected among the individuals in the locations stufied where the species was distributed in terms of morphological characteristics and obvious similarities/differences were determined among the biotopes as a result of the discriminant analysis. Morphological characterization was supported by molecular data. Four individuals from two different locations were analyzed by PCR using twenty-four ISSR primers. Analysis of PCR products on agarose gel yielded three primary reproductive band profiles, and the similarity frequencies calculated with the Jaccard coefficient were found to be among 0.229 and 0.769. In this context, according to the results, there was no difference in species, subspecies or variety level between individuals between the two locations. But the obtained molecular differences support the morphological differences. It is predicted that this may change towards speciation as ecological changes increase in the locations where the species is distributed.
Gypsophila pilulifera Boiss. & Heldr., an endemic and endangered species is an economically important plant that has a potential used in medicine thank to the saponins it contains. It is also an ornamental plant. In this study, the production was carried out under in vitro conditions in order to protect and economically evaluate the species, which is in danger of extinction due to its distribution in a very narrow area open to anthropogenic effects. For this purpose, seed and shoot tip explants of G. pilulifera were cultured in Murashige and Skoog (MS) medium containing different concentrations of 6-benzylaminopurine (BAP), indole-3-acetic acid (IAA) and naphthaleneacetic acid (NAA). Explants were obtained from two different locations of the natural habitats of G. pilulifera and more plants were obtained in the tissue culture from both explant types taken from the 1st location. Propagation from seed gave better results, and 96.67% of the seeds cultured in hormone-free MS medium were germinated and rooted. The shoot tip showed lower regeneration in vitro than the seed explant. Especially the rooting percentage is quite low and the highest rooting and shoot formation was obtained in MS medium containing 2 mg/L BAP + 2 mg/L IAA. In this medium, 10.50 ±0.17 shoots were obtained per plant, and rooting (1.67 ±0.09 per plant) was also achieved only in this medium. Seedlings obtained in tissue culture continue to develop successfully in field conditions, and this method has been determined as a suitable method for ex-situ conservation and economically rapid production of the species.
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