The paper describes the synthesis and characterization of a novel macrocyclic ligand. Liquid-liquid extraction studies were conducted to assess the extraction performance of the new macrobicyclic ligand towards copper ions. Selective extraction of heavy metals is highly demanded due to their toxicity and market significance. The results of the experimental studies to determine the best extraction conditions demonstrates pH=9 in the presence of 8.0 ppm of copper (II) in 10 mL with shaking time equal to 30 minutes at 25 o C with using 0.05% reagent and chloroform as an organic solvent. The article studies the influence of various parameters on the extraction percentage such as; effect of pH, shaking time, type of solvent, temperature, ionic strength, the effect of concentration of metal ion and reagent. IR and HNMR have been used for the characterization of the new macrocyclic compound. Thermodynamic parameters have been calculated based on the experimental results at different temperatures with ΔH (39.91 kJ/ mol), ∆S (0.155 KJ/ mol. K), and ΔG (-6.28 KJ/ mol), which, indicates that the reaction is endothermic, randomness and spontaneous. This process has been used for extracting copper ions using atomic absorption spectrometer (AAS).