Background and objective: Current strategies to address wound care include both traditional and modern options. Medicinal plants, as a part of traditional medicine and photodynamic therapy, as a modern option, have shown promising effects in wound care and repair.In this study, a combination of Plantago major and laser therapy might have more effective wound healing effects was hypothesized. Materials and Methods: To examine this, P. major extract was prepared through the maceration method. Human fibroblasts were harvested in FBS and antibiotic-contained DMEM. Upon reaching 70-80% confluency, cells were seeded in a 96-well plate and then treated with P. major (different concentrations), laser (980 nm) and P. major-laser combination. To determine the cell viability percentage, an MTT assay was performed. A scratch assay to investigate HFFF2 migration after treatment with P. major, laser and their combination was also carried out. The expression of IL-6 and TNF-" as pro-inflammatory cytokines and VEGF as a growth factor were analyzed using a real-time PCR technique. Results: Current findings showed that 160 µg mLG 1 P. major as well as the combination of 160 µg mLG 1 P. major and laser posed no cytotoxicity to HFFF2 cells. Scratch assay results indicated that the P. major and laser combination could effectively increase HFFF2 migration ability as compared to control cells. An upregulated VEGF, IL-6 and TNF-" gene expression in cells treated with the combined P. major and laser was also found. Conclusion: The evidence from this study suggested that the combination of traditional and modern wound repair approaches could result in more favorable outcomes.