Kibale National Park (KNP), Uganda, has a number of chimpanzees habituated for tourism. The close genetic relationship between humans and chimpanzees means that there is the potential for disease transmission between the two. The aim of this study was to establish the diseases to which chimpanzees may be exposed by surveying the medical histories of humans in contact with the chimpanzees of KNP. Medical questionnaires were given to tourists visiting KNP and to the population of a village close to the park. The 62 tourist surveys returned indicated a high prevalence of disease symptoms, in particular diarrhoea, as well as ongoing infectious diseases and a lack of current vaccinations. The 50 local surveys returned also indicated a high prevalence of disease symptoms, in particular respiratory disease, along with a low rate of vaccination and a high frequency of visual contact with the chimpanzees both within and outside KNP. This study indicates that humans are a potential source of infection for chimpanzees. The results, which have been communicated to the appropriate authorities, will assist in the devising of proper tourist viewing regulations and provide local health authorities with the information necessary to improve both public health and chimpanzee health. Further recommendations include education of tourists regarding appropriate vaccinations, hand washing prior to the visit, the use of facemasks during the visit, and the provision of latrines. Chimpanzee ecotourism is becoming increasingly popular and protecting the chimpanzees’ health will help to ensure that ecotourism is a sustainable activity.

Domestic dogs are common amongst communities in sub-Saharan Africa and may serve as important reservoirs for infectious agents that may cause diseases in wildlife. Two agents of concern are canine parvovirus (CPV) and canine distemper virus (CDV), which may infect and cause disease in large carnivore species such as African wild dogs and African lions, respectively. The impact of domestic dogs and their diseases on wildlife conservation is increasing in Zimbabwe, necessitating thorough assessment and implementation of control measures. In this study, domestic dogs in north-western Zimbabwe were evaluated for antibodies to CDV, CPV, and canine adenovirus (CAV). These dogs were communal and had no vaccination history. Two hundred and twenty-five blood samples were collected and tested using a commercial enzyme-linked immunosorbent assay (ELISA) for antibodies to CPV, CDV, and CAV. Of these dogs, 75 (34%) had detectable antibodies to CDV, whilst 191 (84%) had antibodies to CPV. Antibodies to canine adenovirus were present in 28 (13%) dogs. Canine parvovirus had high prevalence in all six geographic areas tested. These results indicate that CPV is circulating widely amongst domestic dogs in the region. In addition, CDV is present at high levels. Both pathogens can infect wildlife species. Efforts for conservation of large carnivores in Zimbabwe must address the role of domestic dogs in disease transmission.

Crop raiding by African elephants (Loxodonta africana) jeopardizes human livelihoods and undermines conservation efforts. Addressing this issue is particularly important in subsistence farms adjacent to protected areas and requires assessing the perceived and actual scale of the problem and the benefits, limitations and adoption potential of mitigation techniques. To achieve these objectives, we assessed the effectiveness of chili and beehive fences relative to control plots, using a daily farm monitoring protocol implemented on 20 farms bordering the Ngorongoro Conservation Area (Tanzania). Prior to the field study, we interviewed 65 farmers about human-elephant interactions and contrasted interview findings with those of daily farm monitoring. Farmer perception of crop raiding frequency declined with increasing distance from the protected area and was, on average, eight times greater than daily farm monitoring data indicated. The majority of interviewees expressed a willingness to try chili or beehive fences, though chili fences were preferred. Generalized-linear-mixed models indicated that neither elephant farm intrusions nor damages were significantly reduced by either chili or beehive fences relative to the control sites. Losses per month and hectare did not differ significantly by fence type. However, farm plots with chili fences did not experience massive damages which occasionally occurred in beehive or control plots. This partial effectiveness of chili fences was further confirmed by contrasting crop losses from a subset of farms that were subject to a cross-over experimental design. Our multidimensional case study suggests that chili fences have greater adoption potential than beehive fences. Nevertheless, additional efforts are required to increase effectiveness and to realize adoption potential. By providing insights into the context and circumstances that presented challenges to the effectiveness, sustainability and scalability of beehive and chili fences, this study can This article is particularly important for conservation practitioners working on implementing methods to mitigate human-elephant conflicts and equally targets scholars who investigate general aspects of human-wildlife conflict and coexistence scenarios.

Feline immunodeficiency virus is a lentivirus of domestic cats that causes significant lifelong infection. Infection with this or similar lentiviruses has been detected in several non-domestic feline species, including African lions (Panthera leo). Although lion lentivirus (FIVple) infection is endemic in certain lion populations in eastern and southern Africa, little is known about its pathogenic effects or its epidemiological impact in free-ranging lions. This report describes the epidemiological investigation of lentivirus positivity of free-ranging lions in the Kruger National Park, South Africa. A nested polymerase chain reaction assay for virus detection was performed on all whole blood samples collected. In addition, serum samples were tested for cross-reactive antibodies to domestic feline lentivirus antigens and to puma lentivirus synthetic envelope peptide antigen. The results were analysed in conjunction with epidemiological data to provide a descriptive epidemiological study on lion lentivirus infection in a free-ranging population of lions. The overall prevalence of lentivirus infection was 69 %, with a prevalence of 41 % in the north of the park, and 80 %in the south. Adult males had the highest prevalence when combining the factors of sex and age: 94 %. The lowest prevalences were found among juveniles, with male juveniles at 29 %. Adults were 5.58 times more likely to test positive for FIVple than juveniles, with adult males being 35 times more likely to be test positive for FIVple compared with juvenile males. This research represents the 1st epidemiological study of the lion lentivirus among free-ranging lions in the Kruger National Park

Background Domestic dogs (Canis familiaris) have the potential to act as disease reservoirs for wildlife and are important sentinels for common circulating pathogens. Therefore, the infectious disease seroprevalence among domestic dogs in northern Botswana may be indicative of pathogen exposure of various wildlife species. The objective of this study was to assess the seroprevalence of Ehrlichia spp., Borrelia burgdorferi, Anaplasma spp., Dirofilaria immitis, canine adenovirus, canine parvovirus, and canine distemper virus in domestic dogs as proxies of disease prevalence in the local wildlife in the Okavango Delta region of Botswana. Statistical analysis assessed crude and factor-specific seroprevalence proportions in relation to age, sex, and geographical location as predictors of seropositivity. Logistic regression was used to identify adjusted predictors of seropositivity for each of the pathogens of interest. Results Samples from 233 dogs in a total of seven locations in Maun, Botswana, and surrounding villages were collected and serologically analyzed. No dogs were seropositive for B. burgdorferi, while low seroprevalence proportions were observed for Anaplasma spp. (2.2%) and D. immitis (0.9%). Higher seroprevalence proportions were observed for the tick-borne pathogen Ehrlichia spp. (21.0%), and 19.7% were seropositive for canine adenovirus (hepatitis). The highest seroprevalence proportions were for canine parvovirus (70.0%) and canine distemper virus (44.8%). The predictors of seropositivity revealed that adults were more likely to be seropositive for canine adenovirus, canine distemper virus, and canine parvovirus than juveniles, and location was a risk factor for canine adenovirus, canine distemper virus, canine parvovirus, and Ehrlichia spp. Conclusions Results indicate that increasing tick control and vaccination campaigns for domestic dogs may improve the health of domestic animals, and potentially wildlife and humans in the