16CRISPR-Cas9-mediated gene interference (CRISPRi) and activation (CRISPRa) 17 approaches hold promise for functional genomic studies and genome-wide screens in 18 human pluripotent stem cells (hPSCs). However, in contrast to CRISPR-Cas9 nuclease 19 approaches, the efficiency of CRISPRi/a depends on continued expression of the dead 20 Cas9 (dCas9) effector and guide RNA (gRNA), which can vary substantially depending 21 on transgene design and delivery. Here, we design new fluorescently labeled piggyBac 22 (PB) vectors to deliver robust and stable expression of multiplexed gRNAs. In addition, 23 we generate hPSC lines harboring AAVS1-integrated, inducible and fluorescent dCas9- 24KRAB and dCas9-VPR transgenes to allow for accurate quantification and tracking of 25 cells that express both the dCas9 effectors and gRNAs. We then employ these systems 26 to target the TCF4 gene and conduct a rigorous assessment of expression levels of the 27 dCas9 effectors, gRNAs and targeted gene. Collectively, these data provide proof-of-28