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MotivationProtein ubiquitination is one of the important post-translational modifications by attaching ubiquitin to specific lysine (K) residues in target proteins, and plays important regulatory roles in many cell processes. Recent studies indicated that abnormal protein ubiquitination have been implicated in many diseases by degradation of many key regulatory proteins including tumor suppressor, oncoprotein, and cell cycle regulator. The detailed information of protein ubiquitination sites is useful for scientists to investigate the mechanism of many cell activities and related diseases.ResultsIn this study we established mUbiSida for mammalian Ubiquitination Site Database, which provides a scientific community with a comprehensive, freely and high-quality accessible resource of mammalian protein ubiquitination sites. In mUbiSida, we deposited about 35,494 experimentally validated ubiquitinated proteins with 110,976 ubiquitination sites from five species. The mUbiSiDa can also provide blast function to predict novel protein ubiquitination sites in other species by blast the query sequence in the deposit sequences in mUbiSiDa. The mUbiSiDa was designed to be a widely used tool for biologists and biomedical researchers with a user-friendly interface, and facilitate the further research of protein ubiquitination, biological networks and functional proteomics. The mUbiSiDa database is freely available at http://reprod.njmu.edu.cn/mUbiSiDa.

A quality evaluation method is an important means and the main basis on which to evaluate the college English classroom teaching quality of teachers. To overcome the one-sided subjectivity and resulting imprecision of the traditional classroom teaching quality evaluation method, a scientific and reasonable quality evaluation index system for college English classroom teaching is constructed. The fuzzy comprehensive evaluation method and the analytic hierarchy process method are combined to propose an improved multi-level fuzzy comprehensive evaluation model for obtaining a new college English classroom teaching quality evaluation method. In the proposed method, according to the fuzzy characteristics of a college English classroom teaching quality evaluation, the fuzzy comprehensive evaluation method is used to transform the qualitative evaluation indexes into limited quantitative evaluation indexes, then a judgment matrix is constructed to determine the weights among different levels by using the analytic hierarchy process method. Additionally, the college English classroom teaching quality is evaluated in detail. Finally, an actual case of college English classroom teaching is used to verify the effectiveness of the college English classroom teaching quality evaluation method. The results show that the proposed college English classroom teaching method can overcome the subjectivity and randomness shortcomings of the traditional classroom teaching quality evaluation methods, and improve the reliability, accuracy, and objectivity of fuzzy comprehensive evaluation. It is an effective method to evaluate college English classroom teaching quality.

In 2020, China suffered novel coronavirus pneumonia epidemic. Under the severe situation, the CPC Central Committee led the whole nation to work together to fight against the epidemic. Teachers and students in the universities should adjust their mentality quickly. Under the premise of good protection, they should make learning plans and make full use of the Internet platform to improve themselves. Universities and teachers should share high-quality curriculum resources and develop a network integrating new educational concepts and educational technology. The rapid development of information technology has a huge impact on people's daily life, work and learning mode. 5g network realizes the interconnection of everything and promotes China to enter the mobile information era. Based on the current complex learning and living background, this paper studies the autonomous learning mode of College English and puts forward the construction strategy of the autonomous learning mode of College English. These measures will serve to cultivate excellent talents for the country under the new challenge.

The applications of the single-hole top lance, the 110-three-holes top lance and the 150-three-holes top lance in the speed of the decarburization are compared. The amount of the Chromium oxidized, and the temperature-increasing speed of the bath is analyzed. The result shows, if the lance gap is set suitably, the 110-three-holes top lance will be the most beneficial to the stainless steel-making in the AOD when the high-carbon pre-metal from the EAF is charged.