POSTERSConclusion: Automastoidectomy is a relatively rare condition of middle ear diseases. It could be caused by cholesteatomatous COM and non-cholesteatomatous COM. Hearing reconstructive procedures are generally not effective compared with other conditions. This study may be helpful to know the clinical features and surgical outcomes of this condition. Otology/NeurotologyClinical Value of VEMP in Ménière's Disease Hye-Youn Youm, MD (presenter); Min Beom Kim; Jeesun Choi; Ha-Young Byun; Ga-Young Park; Won-Ho Chung Objective: 1) To find clinical value of cervical vestibular evoked myogenic potential (VEMP) in Ménière's disease (MD). 2) To evaluate whether the VEMP results can be useful in assessing the stage of MD. 3) To evaluate the clinical effectiveness of VEMP in predicting hearing outcome.

Human papillomavirus type 16 (HPV16) infection is a major risk factor for the development of squamous cell cancers of the cervix and of the head and neck. A major barrier to understanding the progression from initial infection to cancer has been the lack of in vitro models that allow infection, replication, and persistence of the viral genome as an episome in differentiated epithelial cells.Methods: To overcome this barrier, we designed an adenoviral delivery vector that contained a full HPV16 genome flanked by LoxP homologous recombination sites plus a fluorescent reporter that was expressed only after the HPV genome was excised by Cre recombinase.Results: This system delivered circular HPV16 genomes to cervical epithelial cells and well-differentiated human airway epithelia. Following delivery, the HPV16 genome replicated and persisted as an episome in cervical keratinocytes. These cells developed an immortalized phenotype and a dysplastic epithelial appearance. Moreover, induction of differentiation led to the expression of late genes and production of infectious HPV16 virions. Conclusion:This work provides a novel means of introducing biologically active HPV genomes into epithelial cells, which are normally difficult to transfect. These methods will allow the study of HPV genome replication and gene expression in the earliest stages of HPV genome establishment and may provide a means with which to study non-oncogenic HPV viral types.Significance: Understanding the papillomavirus life cycle may aid in developing novel treatments for HPV-related head and neck diseases.

No abstract

Objective: This work aims to link the technological advances (cochlear implant) in prosthetic equipment for the deaf to their impact on voice and speech in profound hearing loss.Method: Thirty-three patients between 6 and 30 years old with bilateral profound sensorineural congenital hearing loss, with cochlear implants. The recorded patient voices have been digitally processed with an Acoustic Analysis of Voice and Speech Program ANAGRAF and have also been analyzed and registered by the segmental and suprasegmentals features of voice and speech.Results: Regarding voice quality, 48% reported a slight improvement and the other 48% a significantly improvement. The remaining 4% did not show changes. A total of 83% of patients tested so far shows a better improvement from personal calibrations between the 1° and 2° sample. Considering all the tested values for voice quality, improvement is observed in almost all subjects tested (32/33). Conclusion:It was observed that patients showing better hearing performance as a result of prosthetic equipment use (cochlear implant), personal adjustment of the acoustic parameters and voice quality in each case, have improved with aspects of vocal quality and articulation precision necessary to achieve good communication. Otology/Neurotology treatment of Hearing Gain in Failed idiopathic Sudden Sensorineural Hearing LossYoung-Seok Choi, PhD (presenter)Objective: To evaluate the effectiveness of intratympanic steroid injection on patients with a history of idiopathic sudden sensorineural hearing loss (ISSNHL) and failure of steroid therapy compared with patients who receive second oral steroids alone.Method: Retrospective review of 70 patients with a history of ISSNHL and failure of hearing gain who receive intratympanic steroid injection or oral steroid as a second treatment modality.Results: Seventy patients met inclusion criteria and were included in the study. The number of patients with improvement in pure tone average in the injection group was 12 (33%) compared with 6 (18%) in the oral group. Fifteen (41.7%) of the injected patients did have improvement in speech discrimination scores of greater than 15% compared with only 4 (11.8%) in the oral group. Conclusion:Patients with ISSNHL who have failed oral steroid therapy can obtain better hearing results with intratympanic steroids injection in comparison to oral steroids. Otology/Neurotology Use of Embolization with Liquid agent in Glomus SurgeryGustavo Rangel (presenter); Marcio Araujo; Luanda Guimarães; Isabelle Mury; Diogo Tavares; Felipe FelixObjective: Evaluate the effects of embolization using liquid embolic agent (Onyx) in surgery for jugulotympanic tumors with regard to length of stay in hospital, duration of procedure, signs of facial palsy, intraoperative hemorrhage, and postoperative complications.Method: Six patients were admitted for evaluation therapeutic options. Two patients were treated with radiotherapy and 4 proceeded to surgery. The selection criteria for the therapeutic choice were staging of the lesion, i...

No abstract