Chestnut is popular worldwide for its unique flavor, high eating quality and nutrition. Here, we evaluated the influence of phosphorylation and acetylation on the structural, physicochemical and functional properties of chestnut starch. Scanning electron micrographs showed the agglomeration of starch granules and the appearance of numerous dents on the starch granule surface under phosphorylation and acetylation. X-ray diffractograms confirmed that the modification treatments did not affect the C-type crystal pattern, but reduced the relative crystallinity of the chestnut starch, particularly phosphorylation. Moreover, modification improved the paste transparency of the starch. Differential scanning calorimeter analysis revealed that the gelatinization temperature and enthalpy of the starch decreased with the increasing substitution degree, particularly in phosphorylated starch. The Rapid Visco Analyser analysis demonstrated that phosphorylation could greatly improve the pasting properties of chestnut starch. In addition, phosphorylated and acetylated starch had a smaller amount of slowly digested starch and a larger amount of resistant starch relative to native chestnut starch. In conclusion, the functional and physicochemical properties of chestnut starch can be significantly improved through phosphorylation and acetylation, demonstrating its great application potential as a food additive.

This study aimed to elucidate the fungal diversity in Changli vineyard soil in China. High-throughput sequencing technology was used to investigate the diversity and composition of soil fungi in five vineyards at different geographical locations of Changli. Although the five vineyards had similar fungal communities, diversity, the composition and distribution of high-abundance species differed. Ascomycota, Basidiomycota and Mortierellomycota were the dominant phyla. Among the14 high-abundance genera of fungi, Odiodendron, Pleotrichocladium and Plectosephalella were rarely reported in other vineyards and are the unique species from Changli region. In addition, Solicoccozyma aeria and Solicoccozyma terrea were the dominant species in five vineyards and were rarely reported in domestic vineyards. Additionally, Rhizophagus, Wardomyces, Mortierella, Volutella and Cryptococcus were significantly different in the five vineyard soils. Among these species, Mortierella was highly abundant in each vineyard, but its contents were significantly different across vineyards. These findings enriched the information on the composition and diversity of soil fungi in the vineyard of Changli region, which helped to explore the regional or distinctive sensorial attributes of wine from the perspective of microbial biogeography.