Objective: To compare the means and changes over time of intra-abdominal fat (IAF) and subcutaneous abdominal fat (SAF) measured by abdominal ultrasound (US) and computerized tomography (CT). Design and Methods: Prospective cohort study of 53 women with obesity and infertility undergoing a lifestyle program. Results: The Pearson's correlation between IAF measurement by US compared to CT was good at baseline, month 3 and 6 (all r ! 0.72). The correlation of SAF measurement by US compared to CT was reasonable at baseline (r ¼ 0.54; 95%CI 0.30-0.78) and weak at month 3 and 6 (all r 0.39). The correlation between the changes in IAF over 3 and 6 months by US compared to CT was reasonable and significant respectively (all r > 0.48). US could not measure the changes of SAF over time. The Bland-Altman plot showed good agreement between US and CT for IAF measurements (À1.1 [95%CI -3.9-1.6] cm lower mean in US) at baseline. For changes of IAF over time, mean estimates were in agreement. Conclusion: In women with obesity and infertility, measuring IAF by US is in good agreement with the CT scan methodology but the measurement of SAF by US is unreliable.