Empathic and moral processing correlates of psychopathic personality have seldom been studied in women. In this study, we experimentally tested associations between psychopathic personality traits and empathic and moral processing in a female sample, and further directly compared them to results from a male sample. We found that high levels of affective-interpersonal traits were associated with diminished affective response to sad and fearful emotions in others; lower propensity to feel moral emotions; higher endorsement of utilitarian solutions to moral dilemmas involving direct harm; and less self-reported difficulty when performing moral decision making. Overall, our findings strengthen the view that the two dimensions of psychopathy make distinct contributions to emotional and moral processing; and demonstrate that, in women, psychopathic personality traits are associated with a similar pattern of empathic and moral processing biases as the one that has been previously reported in men.