No abstract

The soluble form of quinoprotein glucose dehydrogenase (EC 1.1.99.17) from Acinetobacter calcoaceticus has been purified 2430-fold to electrophoretic homogeneity. The purified enzyme shows a specific activity of 2600 units/mg of protein, and 45% of the starting activity is recovered. In the presence of polyethylene glycol 6000 the purified glucose dehydrogenase crystallizes readily. Glucose dehydrogenase possesses a molecular weight of 1 10 000 as determined by sedimentation-equilibrium centrifugation. The