The aim of this study was to investigate the radiosensitizing effects of arsenic trioxide (As2O3) on MCF-7 human breast cancer cells irradiated with 89 strontium chloride (89SrCl2). The 50% inhibitory concentration (IC50) was calculated from results of an MTT assay. The concentration of As2O3 less than 20% IC50 was selected for subsequent experiments. Cells were treated with As2O3 and 89SrCl2. Morphological changes of cells were observed under an inverted microscope. The radiosensitivity enhancing ratio (SER) was computed based on a clone formation assay. Cell cycle distribution and apoptosis were measured by flow cytometry (FCM). Expression of Bcl-2 and Bax at both the mRNA and protein levels was assessed by RT-PCR and western blotting. The IC50 of As2O3 at 24 h was 11.7 µM. Doses of As2O3 (1 and 2 µM) were used in combination treatments and SER values were 1.25 and 1.79, respectively. As2O3 significantly suppressed cell growth, caused G2/M arrest, enhanced cell death and apoptosis induced by 89SrCl2 and decreased expression of the Bcl-2 gene. Since expression of Bax was unchanged following treatment, As2O3 effectively reduced the Bcl-2/Bax ratio. As2O3 (1-2 µM) enhances the cytotoxic effects of 89SrCl2 on the MCF-7 human breast cancer cell line by inducing G2 phase delay and promoting apoptosis through the reduction of the Bcl-2/Bax ratio.
Background:
Textbook outcome (TO) can guide decision-making among patients and clinicians during preoperative patient selection and postoperative quality improvement. We explored the factors associated with achieving a TO for gallbladder carcinoma (GBC) after curative-intent resection and analyzed the effect of adjuvant chemotherapy (ACT) on TO and non-TO patients.
Methods:
A total of 540 patients who underwent curative-intent resection for GBC at the Department of Hepatobiliary Surgery of the First Affiliated Hospital of Xi'an Jiaotong University from January 2011 to December 2020 were retrospectively analyzed. Multivariable logistic regression was used to investigate the factors associated with TO.
Results:
Among 540 patients with GBC who underwent curative-intent resection, 223 patients (41.3%) achieved a TO. The incidence of TO ranged from 19.0% to 51.0% across the study period, with a slightly increasing trend over the study period. The multivariate analysis showed that non-TO was an independent risk factor for prognosis among GBC patients after resection (P = 0.003). Age ≤60 years (P = 0.016), total bilirubin (TBIL) level ≤34.1 μmol/L (P <0.001), well-differentiated tumor (P = 0.008), no liver involvement (P <0.001), and T1–2 stage disease (P = 0.006) were independently associated with achieving a TO for GBC after resection. Before and after propensity score matching (PSM), the overall survival outcomes of non-TO GBC patients who received ACT and those who did not were statistically significant; ACT improved the prognosis of patients in the non-TO group (P <0.05).
Conclusion:
Achieving a TO is associated with a better long-term prognosis among GBC patients after curative-intent resection, and ACT can improve the prognosis of those with non-TO.
Developing multifunctional electronic fabrics with sensing capabilities, electromagnetic interference (EMI) shielding, and Joule heating performance holds great significance for health monitoring and medical protection. However, most fabrics struggle to combine multiple functions and optimize them simultaneously. For example, achieving both high sensitivity and a wide detection range in sensing devices remains a challenge. In this paper, we present a multidimensional composite structure comprising MXene Ti 3 C 2 Tx nano lamellar, self-assembled lotus leaf-like MXene/ EGaIn and carbon nanotubes (CNTs) assembled on the surface of thermoplastic polyurethane to create a multifunctional MXene EGaIn/MXene CNT fabric (MEMC fabric). The multidimensional materials form a stable conductive network under tension. As a strain sensor, the MEMC fabric exhibits a large sensing range (0−360%) and ultra-high sensitivity (GF ∼ 114,700), making it suitable for artificial eardrum research. Additionally, MEMC demonstrates excellent EMI shielding effect (∼73 dB) and maintains good EMI shielding performance under tensile conditions. The fabric also showcases outstanding Joule heating performance (low voltage drive ∼2 V, fast heating time ∼13 s, and high heating stability ∼4000 s). This paper also demonstrates the multifunctional combination of MEMC fabrics, achieving simultaneous sensing, EMI shielding, and Joule heating functions under stretching. This work offers a forward-looking approach for constructing multifunctional composite fabrics, and the resulting MEMC composite materials hold potential applications in portable electronic devices and defense industries.
In this study, a colitis mouse model induced by dextran sulfate sodium (DSS) was used to investigate the mechanisms of action of an extract of crude polysaccharides (POL) from Physalis pubescens L. as a dietary intervention for colitis. Our results showed that the administration of POL prior to DSS‐induced colitis protected the colon mucosal layer; maintained intestinal barrier integrity; alleviated oxidative damage; and lowered neutrophil infiltration by downregulating intercellular cell adhesion molecule‐1 and monocyte chemoattractant protein‐1 expression. More importantly, POL pretreatment reduced the expression of the proinflammatory factors tumor necrosis factor‐α, inducible nitric oxide synthase (iNOS), and cyclooxygenase‐2 (COX‐2), thereby modulating the nuclear factor‐κB/iNOS–COX‐2 signal transduction pathway. In addition, POL reversed DSS‐induced gut dysbiosis, accompanied by reducing the relative abundance of Helicobacter, Mucispirillum, and Erysipelatoclostridium. In conclusion, POL ameliorated DSS‐induced intestinal injury in mice, indicating that POL could be a useful dietary nutrient to protect against colitis.
Practical Application
Physalis pubescens L. is an edible fruit. The results of this study show that the intervention with Physalis pubescens L. crude polysaccharides may help prevent ulcerative colitis.
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