Background Currently, 58 species are assigned to the genus Allobates, with 70% of its diversity described just in the last two decades, with many additional species likely unnamed. The continuous description of these new species represents a fundamental step for resolving the taxonomy and ensuring the future conservation of the genus. Methods Using molecular, acoustic, and morphological evidences, we describe a new species of Allobates from Teles Pires River region, southern Amazonia, and provide accounts on the population of A. tapajos found sympatrically with the new species. Results The new species is distinguished from its congeners by the coloration of thighs, venter, dorsum, and dark lateral stripe. It has four types of calls, with advertisement calls formed by relatively long trills with a mean duration of 2.29 s ± 0.65, mean of 39.93 notes ± 11.18 emitted at a mean rate of 17.49 ± 0.68 notes per second, and mean dominant frequency of 5,717 Hz ± 220.81. The genetic distance between the new species and its congeners in a fragment of the 16S mitochondrial fragment ranged between 13.2% (A. carajas) to 21.3% (A. niputidea). The sympatric Allobates population fits its morphology and acoustic with the nominal A. tapajos, but presents a relatively high genetic distance of nearly 6.5%, raising questions on the current taxonomy of this species.

Based on concordant differences in male advertisement call, tadpole morphology, and absence of haplotype sharing in the barcoding 16S mitochondrial DNA, we describe here a new species of spotted leaf frog of the genus Phasmahyla from Atlantic Forest, State of Rio de Janeiro, Southeast Brazil. The new species is most similar to P. cochranae (type locality) and P. spectabilis (type locality). It differs from these species by the size of the calcar, moderate-sized body (snout-vent length 30.4–34.4 mm in adult eight males), and in the advertisement call. The tadpoles of Phasmahyla lisbella sp. nov. differ from P. exilis, P. spectabilis, P. timbo, P. guttata and P. jandaia because they do not have row of teeth in the anterior part; differ from P. cruzi by the shape of the anterior end of the oral disc. Through genetic data (phylogenetic distance and haplotype genealogy) we diagnosed the new species where the genetic divergences among its congeners is about 3–6% in a fragment of the 16S rRNA gene, which is above the threshold typically characterizing distinct species of anurans. However, the new species can be distinguished from other congeneric species based on an integrative approach (molecular, bioacoustics, larval, and adult morphology).

Lithodytes lineatus is widely distributed in the Amazon and currently found in the Cerrado domain. We document two new records of the species in the transition zone of Amazon-Cerrado located in the state of Tocantins, Brazil. These records extend the geographic distribution of this species and help to fill information gaps in the state of Tocantins, Brazil.

Anurans emit a variety of acoustic signals in different behavioral contexts during the breeding season. The release call is a signal produced by the frog when it is inappropriately clasped by another frog. In the family Phyllomedusidae, this call type is known only for Pithecophus ayeaye. Here we describe the release call of four species: Phyllomedusa bahiana, P. sauvagii, Pithecopus rohdei, and P. nordestinus, based on recordings in the field. The release calls of these four species consist of a multipulsed note. Smaller species of the Pithecopus genus (P. ayeaye, P. rohdei and P. nordestinus), presented shorter release calls (0.022-0.070 s), with higher dominant frequency on average (1508( .8-1651, when compared to the bigger Phyllomedusa (P. bahiana and P. sauvagii) (0.062-0.107 s; 798.7-1071.4 Hz). For phyllomedusid species, the release call might indicate a phylogenetic signal, because species of the same genus have similar acoustic traits.

Ololygon trapicheiroi is a small tree frog of the O. catharinae group which inhabits mountainous areas in the Atlantic Rainforest region of Southeastern Brazil. On 30 April 2017, in the area rural of the Municipality of Passa Vinte, State of Minas Gerais, Brazil, we observed several specimens of O. trapicheiroi. Here we report new records of O. trapicheiroi throughout Minas Gerais, Rio de Janeiro and São Paulo states, Brazil and provide a distribution map of the species.