Pre-surgery is an action before surgery, which can trigger psychological problems, such as anxiety. Spiritual strength is able to help the individual towards the healing and fulfillment of life goals. The aim of the reseach was discover the correlation between the spiritual level and anxiety in preoperative patients in the surgical inpatient units of RSUD Banyumas. This study was conducted from 12 November to 12 December 2019 in the surgical inpatient units of RSUD Banyumas. This research was a non-experimental quantitative, using a correlational research design with a cross-sectional approach. The sample was selected by purposive sampling technique, with 72 sample respondents. The data collection technique used the HARS (Hamilton Rating Scale Anxiety) and DSES (Daily Spiritual Experience Scale questionnaires) questionnaires. The result found 27.8% of patients' preoperative anxiety level at RSUD Banyumas did not experience anxiety, 56.9% felt mild anxiety, and 15.3% suffered from moderate anxiety. Meanwhile, for the spiritual level, only 1.4% of the preoperative patients at RSUD Banyumas had a low spiritual level, 52.8% were at a moderate spiritual level, and 45.8% presented a high spiritual level. The Spearman Rank statistical test obtained p-value = 0.0001; the correlation coefficient was -0.469.  There was a correlation between the patients' spiritual level and preoperative anxiety in the surgical inpatient units of RSUD Banyumas.