is an edible mushroom with significant nutritional properties and highly valuable protein concentrates can be obtained from its fruit bodies. Functional properties of flours and protein concentrates derived from 3 strains (PCM, POS and hybrid PCM × POS) were evaluated in this investigation. Fruit bodies were produced on wheat straw substrate, flours were obtained from dried and grinded fruit bodies and protein concentrates were extracted from flours by alkaline solubilization. For all 3 strains, pale yellow flours were obtained and protein concentrates showed a grayish brown color. Flour bulk densities ranged from 0.52 to 0.64 g/mL, a higher value than those for protein concentrates, i.e. 0.30-0.35 g/mL. The highest water absorption capacities (WAC) were observed for flours (300-418.8%) while protein concentrates presented higher oil absorption capacity (OAC) (173.3-214.1%). Flours and protein concentrates presented a minimal gelation concentration of 2%. Protein concentrates showed a higher foam capacity formation (FC) at pH 8. Likewise, flours and protein concentrates presented higher foam stability (FS) at alkaline pH (8 and 10). Emulsion activity index (EAI) for flours ranged from 3.96 to 26.68 m g whereas for protein concentrates ranged from 1.55 to 10.28 m g. These results indicate that flours and protein concentrates from have remarkable functional properties, valuable in food industry where foaming and emulsifying properties are required.

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A monokaryotization method allows the recovery of the two monokaryotic components (= neohaplonts) from a dikaryon within a sample of few mycelial fragments. This relatively gentle, simple and rapid procedure makes use of glycine containing solutions low in phosphate and magnesium ions. It proved to be successful in all 14 strains (among them two multiple mutants) of the five species tested. No variant or mutant was found among the neohaplonts, not even in a sample of 1018 isolates from a single dikaryon. The method is useful for genetical studies and breeding work with sterile strains.

Modelado de la biodegradación en biorreactores de lodos de hidrocarburos totales del petróleo intemperizados en suelos y sedimentos (Biodegradation modeling of sludge bioreactors of total petroleum hydrocarbons weathering in soil and sediments)