Human papillomavirus (HPV) is the most common sexually transmitted infection worldwide and a necessary condition for cervical cancer (CeCa) development. HPV types 16 and 18 are the most common in cervical lesions (CLs); therefore, specific vaccines have reduced cervical neoplasia. In 2015, HPV16 presented the highest frequency in CLs (~28%); however, types 31, 45, 52, and 58 were also detected, suggesting that other HPV types may have impact on healthcare. Here, we typified 30 patients with CLs from the "Hospital Regional 1°de Octubre" (HR1O), in Mexico City, for the most reported high-risk papillomavirus (HPV16, 18, 31, 33, 45, 58, and 59). The results showed that HPV16 frequency is dissimilar to international and previous national data, suggesting that a deeper knowledge of HPV type distribution is required before choosing a vaccination scheme for HPV in Mexico City.