Background: Helicobacter pylori (H. pylori), one of the most common bacterial pathogens of humans, colonizes the gastric mucosa. H. pylori is a major factor in inflammatory and malignant diseases of the gastrointestinal tract, where it appears to persist throughout the host's life unless the patient is treated. Materials and methods: A study population was carried out through 100 patients who had gastrointestinal symptoms in Hail region. Seroprevalence of Helicobacter pylori was carried out by detection of H. pylori IgG in patient serum by using Enzyme Linked Immunosorbant Assay (ELISA). Results: Among the patients, H. pylori positivity percentage was found to be (57%) (57/100) in our study. It was found that, the average age of the patient was (one to 70 years old), 68% of them were male and 32% female, the majority came from Hail city. Conclusion: high H.pylori positivity ration (57%), that obtained from these study indicates that H.pylori infection is still a common problem among people in Hail region-Saudi Arabia. The titer of IgG antibody to H.pylori in patient serum can be used as non-invasive tests for the presence of gastric H.pylori infection and gastritis.