Abstract:The majority of black Aspergilli, including Aspergillus niger. Here, we provide to evidence that the exploration of sugar uptake in the filamentous fungus A. niger as a sole carbon source. The goal of this research line is determination of the growth of fungi was evaluated every 24h, measuring the colony diameter (cm). A. niger was inoculated onto two culture media: PDA: for maintains a strain as pure while Czapeck Dox Agar was used in investigation into their carbon requirement, using five different carbon sources (vizs. glucose, fructose, sucrose, maltose, and starch). The fungus was tested grew sparsely on the basal medium lacking in carbon, which was the control. However this fungus was found to vary from their ability to use the supplied sources of carbon. Fructose and sucrose were found to be suitable sources of carbon for a fungal isolates, whereas glucose and maltose proved good carbon source to have a higher affinity. Starch as a polysaccharide, was a poor source of carbon for the growth of this isolate. Despite earlier claims, saccharides rather than monosaccharide were breakdowns extracellularly by means of a broad range of extracellular enzyme activities from Aspergillus niger.