Background: In advanced human malignancies, the nuclear apoptosis-inducing factor 1 (NAIF1) is commonly silenced or not expressed. The purpose of this study was to look into potential relationships between clinical, pathological characteristics and NAIF1 expression. Methods: This cross-sectional study used western blot and immunohistochemistry staining to assess the level of NAIF1 expression in 100 colorectal cancer samples. The outcomes of the immunohistochemistry staining were then contrasted with those of the clinicopathological characteristics. Results: 68 out of 100 colorectal cancer samples were negative for NAIF1 expression. In contrast to other clinicopathological parameters, loss of NAIF1 expression was significantly linked with lymphovascular invasion (P=< 0.00001), angiovascular invasion (P= 0.004356) and advanced TNM tumor stage (P=0. 004077). Conclusion: According to the current study, a higher stage and a bad prognosis may be linked to decreased or negative NAIF1 expression. Keywords: Clinical, Colorectal Cancer, Expression, NAIF1, Pathological