Alternative herbal medicines have been used since hundreds of years in globe in treatment of various kinds of infections that may infect human as well as animals. Medicinal plants, in general, contain phytoconstituents some of which having chemical properties resemble to that of synthetic antibiotics. Due to this, the aim of this study is to investigate the antibacterial activity of the green mango (mangifera indica L.) extract against both Gram-negative bacteria and Gram-positive bacteria. The extraction process of the active phytoconstituents of green mango was done by using infusion method. The estimation of antimicrobial activity of green mango extract was carried out by using agar well diffusion method against two species of gram-positive bacteria and five species gram-negative bacteria using three concentrations that are 5%, 10% and 50% of the extract. The 5% concentration of the extract has showed 2 mm diameter inhibition zone against both Proteus and Salmonella, 5mm diameter inhibition zone against Pseudomonas, 7mm diameter against both Klebsiella and Staphylococcus, and 11, 12 mm diameter inhibition zone against E.coli and Streptococcus respectively. The 50% concentration of extract has showed 14 mm diameter against Proteus, 19, 20, 20mm diameter against Salomella, Klebsiella and Staphylococcus respectively, 22, 23 mm diameter against E.coli and Pseudomonas respectively and 26 mm diameter against Streptococcus. Lastly, the 10 % concentration of extract has showed a result ranged between the 5% concentration and 50% concentration.

Background: The immune response against Blastomyces dermatiditis is less clear than that of Histoplasma spp. or Coccidioides spp. This study was done to evaluate the cellular immune host response in patients with ulcerative and verrucous skin lesions. Methods: This study was conducted on 50 patient attended Al-Kadhymiah Teaching Hospital/Dermatology Dep. in Baghdad (2009Baghdad ( -2010. After processing of skin samples, all cases were studied for various lymphocyte subtypes by using direct dual immunofluorescence staining method. Independent sample t-test was used to make a comparison between groups and p-value <0.05 was considered significant. Results: Histopathological examination showed 4 biopsies of skin lesions were Blastomyces dermatitidis positive and 46 biopsies were negative, two cases were ulcerative and two were verrocous . According to this classification, the total cell counts, CD3+ cells was found statistically different (p=0.034). Plasma cell (CD19), T-helper (CD4) and T-cytotoxic (CD8) also were significant when p values were (p=0.046), (p=0.020) and (p=0.018) respectively. According to the type of lesion whether it is ulcerative or verrocous, CD3+, CD4+, CD8+ and CD19+ counts for each type were significant when, P value were (p≤0.001, p=0.003, p=0.026 and 0.050) respectively. However, there were no significant difference was seen for these cells (p>0.05) among negative lesions.