Ceramide generation and invasive inhibition by hybrid liposomes (HL) composed of 90 mol% L-α-dimyristoylphosphatidylcholine (DMPC) and 10 mol% polyoxyethylene (23) dodecyl ether (C 12 (EO) 23 ) were examined for human lung cancer (A549) cells in vitro. Ceramide generation for A549 cells treated with HL was observed through the activation of neutral sphingomyelinase. These was a reduction in sphingomyelin in A549 cells after treatment with HL. The anti-invasive effects of HL for A549 cells were obtained through the inhibition of MT1MMP/MMP14. Inhibitory effects of HL on the migration of A549 cells was obtained using scratch assay.