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Administration of a sublethal dose of lead acetate to rats induced the simultaneous synthesis of a Pb-metallothionein (Pb-MT)-like protein (Pb-BP) and Zn-thionein (Zn-BP) in the liver. The Pb-BP had an apparent molecule mass of 6900 Da and seemed to bind preferentially to lead in the liver cytosol. The Zn-BP was identified by comparison of the Mr, elution profiles from Sephadex G-75 and DEAE-Sephadex A-25 columns, and polyacrylamide-gel-electrophoretic mobility, with those of rat liver Zn-MT-II. The Pb-BP accumulated in the liver to a maximum 6 h after the intraperitoneal injection of lead acetate and accounted for about 60% of the lead in the liver cytosol at this stage. However, after that, it gradually decreased in the liver, until it was close to the basal amount 24 h after the induction. In contrast, the amount of Zn-MT increased gradually, reached a maximum 12 h after the administration of lead acetate and maintained a constant value until at least 24 h after the induction. Amino acid analysis of the Pb-BP indicated that it contained about 280% half-cysteine. These results strongly suggest that lead acetate induces the synthesis of Pb-MT as well as Zn-MT in rat liver.

An easy, sensitive, competitive indirect enzyme-linked immunosorbent assay (CI-ELISA) for zearalenone in barley and Job's-tears was established. To improve sensitivity of the assay system for zearalenone, a solid phase antigen was prepared by affinity purification. This assay system uses a purified antigen and specific monoclonal antibodies for zearalenone. Consequently, the detection limit of zearalenone by CI-ELISA was increased to 0.2 ng/mL (equivalent to 10 ng/g in barley and Job's-tears). Zearalenone in barley and Job's-tears samples could be determined in the range of 25-1000 ng/g using method A (methanol-water and dichloromethane extraction) and method B (methanol-water alone) as sample preparation. The average recoveries of zearalenone from barley samples were observed to be 106% (mean CV, 10.3%) by method A and 98% (mean CV, 7.5%) by method B; also, those of zearalenone from Job's-tears samples were observed to be 96% (mean CV, 9.4%) by method A and 102% (mean CV, 6.5%) by method B. There was little or no difference between method A and method B for the recovery. For the benefit of a facile sample preparation, unknown amounts of zearalenone in barley and Job's-tears samples were assayed by method B. The results obtained by CI-ELISA were closely correlated with those of high-performance liquid chromatography.

Here we present the guideline for the treatment of neuroendocrine tumors using Lu-177-DOTA-TATE on the basis of radiation safety aspects in Japan. This guideline was prepared by a study supported by Ministry of Health, Labour, and Welfare, and approved by Japanese Society of Nuclear Medicine. Lu-177-DOTA-TATE treatment in Japan should be carried out according to this guideline. Although this guideline is applied in Japan, the issues for radiation protection shown in this guideline are considered internationally useful as well. Only the original Japanese version is the formal document.

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