In order to investigate the relationship between bitterness and chemical structure of BPIa, eleven kinds of fragments and analogs of BPIa were synthesized. des–Gly2–BPIa and des-Pro4-BPIa exhibited extremely bitter taste. However, the pentapeptide (Arg–Gly–Pro–Pro–Phe) of BPIa possessed weak bitterness. The bitterness exhibition of BPIa probably derived from the spatial structure of its molecule.
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