Hyperthermostable -glucosidase from Pyrococcus furiosus was enclosed in gelatin gel by cross-linking with transglutaminase. Gelatin-immobilized -glucosidase was considerably more thermostable than the native enzyme. Lyophilized immobilisate was stored at 90 C for 1 month without loss of activity. The immobilized -glucosidase catalyzed transglucosylation of 5-phenylpentanol with 10.0 equivalent of cellobiose at pH 5.0 and 70 C for 12 h to afford 5-phenylpentyl -Dglucopyranoside in 41% yield. The immobilized enzyme was more effective than the native one in transglucosylation. The gelatin-immobilized Pfu--glucosidase recovered from the first run of the reaction was reusable on successive runs.

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Carbohydrates U 0500Synthesis of Alkyl β-Glucosides by Transglucosylation Using Pyrococcus furiosus β-Glucosidase Immobilized onto Gelatin Gel. -Hyperthermostable β-glucosidase immobilized onto gelatin gel is applied for the transglucosylation between alcohols (II) and cellobiose (I) as glucosyl donor. -(MATSUSHITA*, Y.-I.; NAGATOMO, H.; SUGAMOTO, K.; MATSUI, T.; Lett. Org. Chem. 6 (2009) 2, 115-119; Dep. Appl. Chem., Fac. Eng., Miyazaki Univ., Gakuen, Miyazaki 889-21, Japan; Eng.) -R. Staver 32-187

Biochemical synthesesBiochemical syntheses O 0035 Enantioselective Reduction of γ-Hydroperoxy-α,β-unsaturated Carbonyl Compounds Catalyzed by Lipid-Coated Peroxidase in Organic Solvents. -(NAGATOMO, H.; MATSUSHITA*, Y.-I.; SUGAMOTO, K.; MATSUI, T.; Tetrahedron: Asymmetry 14 (2003) 16, 2339-2350; Fac. Eng., Miyazaki Univ., Gakuen, Miyazaki 889-21, Japan; Eng.) -S. Adam 49-037