One of the advantages of the X-ray Imaging Spectrometer (XIS) system on board Suzaku is its low and stable non-X-ray background (NXB). In order to make the best use of this advantage, modeling the NXB spectra with high accuracy is important to subtract them from the spectra of on-source observations. We construct an NXB database by collecting XIS events when the dark Earth covers the XIS FOV. The total exposure time of the NXB data is about 785 ks for each XIS. It is found that the count rate of the NXB anti-correlates with the cut-off-rigidity and correlates with the count rate of the PIN upper discriminator (PIN-UD) in Hard X-ray Detector on board Suzaku. We thus model the NXB spectrum for a given on-source observation by employing either of these parameters and obtain a better reproducibility of the NXB for the model with PIN-UD than that with the cut-off-rigidity. The reproducibility of the NXB model with PIN-UD is 4.55-5.63 % for each XIS NXB in the 1-7 keV band and 2.79-4.36 % for each XIS NXB in the 5-12 keV band for each 5 ks exposure of the NXB data. This NXB reproducibility is much smaller than the spatial fluctuation of 1 arXiv:0803.0616v1 [astro-ph] 5 Mar 2008 the cosmic X-ray background in the 1-7 keV band, and is almost comparable to that in the 5-12 keV band.

A novel enzyme immunoassay (immune complex transfer enzyme immunoassay) for anti-thyroglobulin IgG using beta-D-galactosidase from Escherichia coli as label was reported previously. This immunoassay was highly sensitive in demonstrating anti-thyroglobulin IgG not only in all patients with Graves' disease and chronic thyroiditis but also in a large proportion of healthy subjects. However, the detection of anti-thyroglobulin IgG at low levels in some serum samples was difficult, probably due to the presence of anti-beta-D-galactosidase antibodies. In the present study, the use of inactive beta-D-galactosidase was tested for elimination of interference by anti-beta-D-galactosidase antibodies. Preincubation of serum samples with excess of inactive beta-D-galactosidase resulted in sufficiently low backgrounds to detect low levels of anti-thyroglobulin IgG with little effect on the dose-response of anti-thyroglobulin IgG. As a result, it was revealed that anti-thyroglobulin IgG was present in almost all healthy subjects as well as all patients with Graves' disease and chronic thyroiditis.

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The ultrasensitive TgAb EIA was useful for detecting the physiological changes in autoantibody formation in healthy subjects and the TgAb value was useful for predicting post-partum thyroid dysfunction in autoimmune thyroid diseases. This EIA is useful for the evaluation of the immune surveillance in patients with autoimmune thyroid diseases as well as in healthy subjects.

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